A direct staining technique for amylase isoenzyme demonstration.

A direct staining technique for amylase isoenzyme demonstration.

Remazolbrilliant Blue covalently linked to amylose (Amylose Azure) has been used for the determination of ex-amylase (Rinderknecht, Wilding, and Haverback, 1967). It forms an insoluble suspension in aqueous media, but liberates a soluble highly coloured blue dye as a result of amylase activity. This communication describes the use of this compound for the demonstration of amylase isoenzymes sep...

Introducing a combined Leishman-Giemsa stain as a new staining technique for avian blood smears

Background: The use of conventional stains including Giemsa, Wright and Leishman have become an essential tool for differential diagnosis of cells in peripheral blood. Aims: The aim of the study was to develop a new combination of Leishman-Giemsa (L&G;) stain for avian blood smears and to compare its efficacy with conventional staining methods. Methods:<...

A new staining technique for polar bodies.

Golgi Staining Technique

The Golgi staining technique, also called the black reaction after the stain's color, was developed in the 1870s and 1880s in Italy to make brain cells (neurons) visible under the microscope [3]. Camillo Golgi developed the technique while working with nervous tissue, which required Golgi to examine cell structure under the microscope [3]. Golgi improved upon existing methods of staining, enabl...

A modified technique for high-resolution staining of myelin.

This report describes a new modification of the Gallyas method for staining myelin in fixed brain tissue and compares results of multiple myelin-visualizing techniques in normal common marmoset (Callithrix jacchus), normal macaque monkey (Macaca mulatta), and a human with multiple sclerosis. The new modification involves immersion in 10% formalin following impregnation in ammoniacal silver nitr...